Authors: Kahui Lim, Matthew Rolston, Samantha Barnum...

In this study, we examined the total bacterial community associated with ureolytic biomineralization from urine drainage systems. Biomineral samples were obtained from 11 California Department of Transportation public restrooms fitted with waterless, low-flow, or conventional urinals in 2019. Following high throughput 16S rRNA Illumina sequences processed using the DADA2 pipeline, the microbial diversity assessment of 169 biomineral and urine samples resulted in 3,869 reference sequences aggregated as 598 operational taxonomic units (OTUs). Using PERMANOVA testing, we found strong, significant differences between biomineral samples grouped by intrasystem sampling location and urinal type. Biomineral microbial community profiles and alpha diversities differed significantly when controlling for sampling season. Observational statistics revealed that biomineral samples obtained from waterless urinals contained the largest ureC/16S gene copy ratios and were the least diverse urinal type in terms of Shannon indices. Waterless urinal biomineral samples were largely dominated by the Bacilli class (86.1%) compared to low-flow (41.3%) and conventional samples (20.5%), and had the fewest genera that account for less than 2.5% relative abundance per OTU. Our findings are useful for future microbial ecology studies of urine source-separation technologies, as we have established a comparative basis using a large sample size and study area.

Topics: Bacteria; Bathroom Equipment; Biomineralization; California; DNA, Bacterial; Drainage, Sanitary; High-Throughput Nucleotide Sequencing; Humans; Microbiota; Phylogeny; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Toilet Facilities

PubMed: 35030221
DOI: 10.1371/journal.pone.0262425

Authors: Utsav Thapa, Priyamvada Sharma, David Hanigan...

Prior measurements at bench scale revealed that waterless urinal cartridges containing oily sealant fluids are capable of partitioning pharmaceuticals from urine and therefore reducing their concentration in wastewater. We sought to measure pharmaceutical removal from in-use waterless urinals. We developed a method to quantify pharmaceuticals in the sealant phase, which resulted in 79 ± 30% and 71 ± 30% recovery of eight pharmaceuticals from two sealant fluids, respectively. The method was applied to sealant samples collected over three weeks from in-use waterless urinals on a university campus. Six of eight pharmaceuticals were present in the sealant samples from 1.4 µg/L to 241 µg/L. Loads of the six pharmaceuticals detected in the sealants were removed from the receiving wastewater from 0.02 µg/day to 3.4 µg/day across the sampling period. The concentration of the pharmaceuticals were similar over time, indicating rapid saturation and washout of the sealant. We also observed relatively rapid loss of sealant at maintenance intervals consistent with the manufacturer's instructions. These findings indicate that while waterless urinals do remove some pharmaceuticals from the wastewater stream, meaningful changes to wastewater concentrations will only result if the sealant fluid and/or the urinal cartridge are significantly modified. PRACTITIONER POINTS: We developed a quantification method for pharmaceuticals in oily waterless urinal sealants. Pharmaceuticals were present at relatively low concentrations in the sealant phase of two in-use waterless urinals. We identify engineering challenges that must be overcome to meaningfully reduce pharmaceutical loads in wastewater with waterless urinals.

Topics: Bathroom Equipment; Environmental Monitoring; Humans; Pharmaceutical Preparations; Waste Disposal, Fluid; Waste Water; Water Pollutants, Chemical

PubMed: 34153156
DOI: 10.1002/wer.1600

Authors: Zhen Ding, Hua Qian, Bin Xu...

Respiratory and fecal aerosols play confirmed and suspected roles, respectively, in transmitting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). An extensive environmental sampling campaign of both toilet and non-toilet environments was performed in a dedicated hospital building for patients with coronavirus disease 2019 (COVID-19), and the associated environmental factors were analyzed. In total, 107 surface samples, 46 air samples, two exhaled condensate samples, and two expired air samples were collected within and beyond four three-bed isolation rooms. The data of the COVID-19 patients were collected. The building environmental design and the cleaning routines were reviewed. Field measurements of airflow and CO concentrations were conducted. The 107 surface samples comprised 37 from toilets, 34 from other surfaces in isolation rooms, and 36 from other surfaces outside the isolation rooms in the hospital. Four of these samples were positive, namely two ward door handles, one bathroom toilet seat cover, and one bathroom door handle. Three were weakly positive, namely one bathroom toilet seat, one bathroom washbasin tap lever, and one bathroom ceiling exhaust louver. Of the 46 air samples, one collected from a corridor was weakly positive. The two exhaled condensate samples and the two expired air samples were negative. The fecal-derived aerosols in patients' toilets contained most of the detected SARS-CoV-2 in the hospital, highlighting the importance of surface and hand hygiene for intervention.

Topics: Bathroom Equipment; Betacoronavirus; COVID-19; Coronavirus Infections; Hospitals; Humans; Pandemics; Pneumonia, Viral; SARS-CoV-2; Severe Acute Respiratory Syndrome

PubMed: 32891988
DOI: 10.1016/j.scitotenv.2020.141710

Authors: Utsav Thapa, David Hanigan

We investigated the potential for waterless urinal sealants fluids to remove pharmaceuticals from urine. H NMR, FTIR, and GC/MS characterization of the fluids indicated that they are mostly composed of aliphatic compounds. Removal of ethinyl estradiol was >40% for two of the three sealant fluids during simulated urination to a urinal cartridge but removal of seven other compounds with greater hydrophilicity was <30%. At equilibrium with Milli-Q water, ≥ 89% partitioning to the sealant phase was observed for three compounds with pH adjusted log (log ) > 3.5. At equilibrium with synthetic urine, removal ranged widely from 2% to 100%. was poorly correlated with removal for both matrices at equilibrium, but was correlated with removal from synthetic urine for two of the three sealants, indicating that ionization and hydrophilicity control partitioning between the urine and sealant phases. To improve removal during urination, where equilibrium is not achieved, we increased the hydraulic retention time 100-fold over that of typical male urination. Removal of specific hydrophobic compounds increased, indicating that both hydrophobicity and kinetics control removal. Removal of ethinyl estradiol was ≥90% for all sealants in the increased hydraulic retention time experiment, demonstrating the potential for implementation to female urinals.

Topics: Bathroom Equipment; Gas Chromatography-Mass Spectrometry; Hydrophobic and Hydrophilic Interactions; Kinetics; Water

PubMed: 32321246
DOI: 10.1021/acs.est.9b06205

Authors: Zhongyan Lu, Hong Shen, Zanming Shen...

Two experiments were performed in this study. In Experiment 1, twenty goats were fed with an isonitrogenous diet, containing 28% Non-Fiber Carbohydrate (MNFC group, = 10) or 14% NFC (LNFC group, = 10). In the MNFC group, the ruminal concentration of Short Chain Fatty Acids (SCFA) increased, and pH declined. Compared with those in the LNFC group, the microbial protein synthesis in rumen and mRNA abundance of urea transporter B (UT-B) in rumen epithelium increased in the MNFC group, although serum urea-N (SUN) did not differ significantly between groups. Simultaneously, urinal urea-N excretion was reduced in the MNFC group. Significant correlations were found between rumen SCFA and UT-B and between UT-B and urinal urea-N excretion. Furthermore, the abundances of SCFA receptor of GPR41 and GPR43 increased in the rumen epithelium of the MNFC group. These results suggest that increases of SUN transported into the rumen and incorporated into microbial protein and decreases of urinal urea-N excretion are related to ruminal SCFA. This is supported by data from our previous study in which added SCFA on the mucosal side caused increases of urea transport rate (flux J urea) from the blood to the ruminal lumen side. In Experiment 2, we used 16S rRNA Amplicon Sequencing to analyze the structure of the ruminal microbiota community in relation to SCFA. An additional eight goats were assigned into the MNFC ( = 4) and LNFC ( = 4) groups. The dietary ingredients, chemical composition, and feeding regimes were the same as those in Experiment 1. Constrained correspondence analysis (CCA analysis) revealed NFC promoted the expansion of microbiota diversity, particularly of SCFA-producing microbes. The function prediction of 19 upregulated Kyoto Encyclopedia of Genes and Genomes (KEGG) ortholog groups showed an NFC-induced increase of the types and abundances of genes coding for enzymes catalyzing N and fatty acid metabolism. Based on our present and previous investigations, our results indicate that, in goats consuming a MNFC diet, the facilitated urea transport in the rumen and improved urea N salvage are triggered by an expansion of ruminal microbiota diversity and are signaled by ruminal SCFA. This study thus provides new insights into the microbiota involved in the dietary modulation of urea-N salvage in ruminant animals.

PubMed: 31507445
DOI: 10.3389/fphys.2019.01079

Authors: Xiaowen Hu, Wei Ni, Zhaoguo Wang...

Roles of environmental factors in transmission of COVID-19 have been highlighted. In this study, we sampled the high-touch environmental surfaces in the quarantine room, aiming to detect the distribution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the environmental surfaces during the incubation period of coronavirus disease 2019 (COVID-19) patients. Fifteen sites were sampled from the quarantine room, distributing in the functional areas such as bedroom, bathroom and living room. All environmental surface samples were collected with sterile polyester-tipped applicator pre-moistened in viral transport medium and tested for SARS-CoV-2. Overall, 34.1% of samples were detected positively for SARS-CoV-2. The positive rates of Patient A, B and C, were 46.2%, 0% and 61.5%, respectively. SARS-CoV-2 was detected positively in bedroom and bathroom, with the positive rate of 50.0% and 46.7%, respectively. In contrast, living room had no positive sample detected. Environmental contamination of SARS-CoV-2 distributes widely during the incubation period of COVID-19, and the positive rates of SARS-CoV-2 on environmental surfaces are relatively high in bathroom and bedroom.

Topics: Bathroom Equipment; COVID-19; Disinfection; Environmental Microbiology; Environmental Pollution; Female; Humans; Infectious Disease Incubation Period; Latent Infection; Male; Quarantine; SARS-CoV-2; Surface Properties; Toilet Facilities

PubMed: 33039873
DOI: 10.1016/j.ecoenv.2020.111438

Authors: Rajanbir Kaur, Drishtant Singh, Anup Kumar Kesavan...

BACKGROUND

The present study was carried out to investigate the tap water quality of public toilets in Amritsar, Punjab, India.

METHODS

Water samples from the taps of the public toilets were collected in sterile containers and physicochemical and bacteriological analysis was performed using standard methods. Also, genotypic and phenotypic characterization of the bacterial isolates was performed using different biochemical tests and 16S ribosomal RNA analysis. An antibiotic susceptibility test was performed using antibiotics based on their mode of action. A biofilm assay was performed to assess the adhesion potential of the isolates.

RESULTS

A total of 25 bacterial isolates were identified from the water samples, including Acinetobacter junii, Acinetobacter pittii, Acinetobacter haemolyticus, Bacillus pumilus, Bacillus megaterium, Bacillus marisflavi, Bacillus flexus, Bacillus oceanisediminis, Pseudomonas otitidis, Pseudomonas sp. RR013, Pseudomonas sp. RR021, Pseudomonas sp. RR022, Escherichia coli and Enterobacter cloacae. The results of the antimicrobial susceptibility test revealed that the antibiotics cefodroxil, aztreonam, nitrofurantoin, cefepime, ceftazidime and amoxyclav were found to be mostly ineffective against various isolates. The biofilm assay revealed the weak, moderate and strong biofilm producers among them.

CONCLUSIONS

The tap water in the public toilets was microbially contaminated and needs to be monitored carefully. The antibiotic susceptibility profile showed that of 25 bacterial isolates, 5 were multidrug resistant. Bacterial isolates exhibited strong to weak adhesion potential in the biofilm assay.

Topics: Acinetobacter; Anti-Bacterial Agents; Bacillus; Bacterial Infections; Bathroom Equipment; Biofilms; Genotype; Humans; India; Microbial Sensitivity Tests; Phenotype; Pseudomonas; Water; Water Microbiology

PubMed: 31693132
DOI: 10.1093/inthealth/ihz074

Authors: Alberto Donzelli, Giulia Giudicatti

Systematic reviews have shown a prevalence close to 20% of gastrointestinal symptoms in COVID-19 positive patients, with nearly 40% of patients shedding viral RNA in their faeces, even if it may not be infectious, possibly because of inactivation by colonic fluid.According to current evidence, this virus is primarily transmitted by respiratory droplets and contact routes, including contaminated surfaces. The virus is quite stable on stainless steel, being detected up to 48-72 hours after application. Therefore, some individuals can be infected touching common contaminated surfaces, such as bathroom taps. Taps can be underestimated critical points in the transmission chain of the infection. Indeed, just by turning the knob, people leave germs on it, especially after coughing over their hands, sneezing, and/or blowing their nose. After handwashing with soap, user take back their germs when turning the knob. Paradoxically, the following user collects the germs back on his/her fingers by implementing a preventive measure, maybe before putting food into the mouth or wearing contact lenses.The Italian National Institute of Health recommends to clean and disinfect high-touched surfaces, but it is unrealistic and inefficient to do so after each tap use. As an alternative, new toilets should install long elbow-levers - or at least short levers - provided that people are educated to close them with the forearm or the side of the hand. This is already a standard measure in hospitals, but it is particularly important also in high-risk communities, such as retirement homes and prisons. It would be important also in schools, in workplaces, and even in families, contributing to the prevention both of orofaecal and respiratory infections.In the meantime, people should be educated to close existing knobs with disposable paper towel wipes or with toilet paper sheets.

Topics: Bathroom Equipment; COVID-19; Equipment Contamination; Equipment Design; Feces; Female; Fomites; Hand Hygiene; Health Education; Humans; Italy; Male; SARS-CoV-2; Touch

PubMed: 33412826
DOI: 10.19191/EP20.5-6.S2.091

Authors: Matthew Olsen, Anna Lohning, Mariana Campos...

An ever-increasing number of medical staff use mobile phones as a work aid, yet this may pose nosocomial diseases. To assess and report via a survey the handling practices and the use of phones by paediatric wards healthcare workers. 165 paediatric healthcare workers and staff filled in a questionnaire consisting of 14 questions (including categorical, ordinal and numerical data). Analysis of categorical data used non-parametric techniques such as the Chi-squared test. Although 98% of respondents (165 in total) report that their phones may be contaminated, 56% have never cleaned their devices. Of the respondents that clean their devices, 10% (17/165) had done so with alcohol swabs or disinfectant within that day or week; and an additional 12% respondents (20/165) within that month. Of concern, 52% (86/165) of the respondents use their phones in the bathroom, emphasising the unhygienic environments in which mobile phones/smartphones are constantly used. Disinfecting phones is a practice that only a minority of healthcare workers undertake appropriately. Mobile phones, present in billions globally, are therefore Trojan Horses if contaminated with microbes and potentially contributing to the spread and propagation of micro-organisms as per the rapid spread of SARS-CoV-2 virus in the world.

Topics: Bathroom Equipment; COVID-19; Cell Phone; Cross Infection; Delivery of Health Care; Disinfection; Emergency Service, Hospital; Female; Hand Hygiene; Hospitals, Pediatric; Humans; Intensive Care Units, Neonatal; Male; Personnel, Hospital; Risk Factors; SARS-CoV-2; Self Report

PubMed: 34155278
DOI: 10.1038/s41598-021-92360-3

Authors: Tesfay Tsegay Gebru, Yohannes Ashebir Tesfamichael, Muzayene Tilahun Bitow...

OBJECTIVE

The objective of the study was to assess the prevalence of stunting and associated factors among under-five children of Wukro town, Tigray, Ethiopia, 2017-2018.

RESULT

Totally 394 under-five children were participated in this study with a response rate of 98.5%. A total of 222 (56.3%) of respondents were females and 106 (26.95%) were in the age group of 12-23 month. One hundred ninety-eight (50.3%) of the participants were between 2 and 3 in birth order and 194 (49.2%) had 4 to 5 house hold size. The overall prevalence of stunting was 194 (49.2%). Being female and presence of washing facilities nearby latrine were significantly associated with stunting. Under-five female children were 35.4% lower odds of stunting compared to male children (p = .041, OR = .644, and 95% CI (.422, .983)).

Topics: Bathroom Equipment; Child; Cross-Sectional Studies; Ethiopia; Female; Growth Disorders; Hand Hygiene; Humans; Infant; Male; Malnutrition; Prevalence; Risk Factors; Sex Factors; Socioeconomic Factors; Surveys and Questionnaires

PubMed: 31412922
DOI: 10.1186/s13104-019-4535-2